A cyclonic separation device is provided having improved pressure loss characteristics. A fluid supply conduit (46) in flow communication with an inlet (56) to a cyclone (28) is located and configured to extend longitudinally through a central portion of the cyclone (28), whereby the sharp bending of the conduit (56) may be reduced, thereby reducing pressure losses in the device, without unduly interfering with the cyclonic flow within the cyclone (28). The present invention may be adapted for use with cyclonic separation devices of all types, including single - and multi-stage cyclonic separators. The cyclonic separation device may be incorporated in a vacuum cleaner such as an upright vacuum cleaner. A three dimensional conduit (56) which is optionally used with a cyclonic separator is provided.