发明公开
- 专利标题: PHAGE-DEPENDENT SUPERPRODUCTION OF BIOLOGICALLY ACTIVE PROTEIN AND PEPTIDES
- 专利标题(中): 噬菌体依赖超强的生物活性肽和蛋白质
-
申请号: EP01964014.3申请日: 2001-08-15
-
公开(公告)号: EP1309604A2公开(公告)日: 2003-05-14
- 发明人: KORDYUM, Vitaliy, A. , CHERNYKH, Svitlana, I. , SLAVCHENKO, Iryna, Yu , VOZIANOV, Oleksandr, F.
- 申请人: Phage Biotechnology Corporation
- 申请人地址: Suite 100,14272 Fanklin Avenue Tustin, CA 92780 US
- 专利权人: Phage Biotechnology Corporation
- 当前专利权人: Phage Biotechnology Corporation
- 当前专利权人地址: Suite 100,14272 Fanklin Avenue Tustin, CA 92780 US
- 代理机构: De Clercq, Ann
- 优先权: US225437P 20000815
- 国际公布: WO02014468 20020221
- 主分类号: C07H21/04
- IPC分类号: C07H21/04 ; C12P21/00
摘要:
This invention relates to a method for enhancing the production of biologically active proteins and peptides in bacterial cells by infecting bacterial cells of the producer strain, which contain a plasmid with one or more targeted genes, with bacteriophage μ with or without the targeted gene(s). The targeted genes encoding the biologically active proteins are under the control of a T7 polymerase promoter and the bacteria also are capable of expressing the gene for T7 RNA polymerase. The phage increases synthesis of the targeted protein and induces lysis of the producer strain cells. Super-production is achieved by the combination of the high level of expression achieved from the T7 polymerase promoter and by cultivating the producer strain cells under culture conditions that delay lytic development of the phage. The biologically active proteins and peptides subsequently accumulate in a soluble form in the culture medium as the cells of the producer strain are lysed by the phage.
公开/授权文献
信息查询