发明公开
- 专利标题: GENE ANALYSIS METHOD AND ANALYZER THEREFOR
- 专利标题(中): 基因分析方法及其分析仪
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申请号: EP02705325.5申请日: 2002-03-19
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公开(公告)号: EP1375673A1公开(公告)日: 2004-01-02
- 发明人: KONDO, Toshihiko , ABE, Shuzo c/o GL Sciences Inc. Technical Center , SHIKAMA, Nobuyosi c/o GL Sciences Inc. Techn.Ctr. , SHINTANI, Yukihiro c/o GL Sciences Inc.Techn.Ctr.
- 申请人: Japan Science and Technology Corporation , Gl Sciences Incorporated
- 申请人地址: 4-1-8 Hon-cho Kawaguchi-shi, Saitama-ken 332-0012 JP
- 专利权人: Japan Science and Technology Corporation,Gl Sciences Incorporated
- 当前专利权人: Japan Science and Technology Corporation,Gl Sciences Incorporated
- 当前专利权人地址: 4-1-8 Hon-cho Kawaguchi-shi, Saitama-ken 332-0012 JP
- 代理机构: Schmid, Wolfgang
- 优先权: JP2001095419 20010329; JP2002063943 20020308
- 国际公布: WO2002079510 20021010
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12M1/00
摘要:
This invention relates to a method for analyzing a gene and an analysis device thereof which are suited to be used, for example, as a fully automatic gene analysis device or a fully automatic gene diagnostic device which can be simplified in structure, made compact in size and light in weight, and manufactured at a low cost and in which detection of various kinds of DNA can be conducted rapidly and with precision and analysis of a plurality of genes can be made simultaneously from different kinds of samples by restraining, as much as possible, the use of a valve in an analytic system, thereby preventing admixture of cross contamination.
It relates to a gene analysis method comprising the steps of extracting a target nucleic acid from a biological sample (23) and amplifying a target DNA, thereafter, introducing a reaction eluent (L) containing a predetermined DNA into a stationary-phase DNA probe (53) having a predetermined temperature and arranged in series or in parallel and separating a DNA complementary to the stationary-phase DNA probe (53).
The above-mentioned gene analysis method comprises a plurality of stationary-phase DNA probes (64 through 66) at least a part of which can be set to a temperature for forming a double strand of DNA to be tested and a reaction eluent containing the same or different kinds of DNA which have been amplified, is introduced into the stationary-phase DNA probes (64 through 66).
It relates to a gene analysis method comprising the steps of extracting a target nucleic acid from a biological sample (23) and amplifying a target DNA, thereafter, introducing a reaction eluent (L) containing a predetermined DNA into a stationary-phase DNA probe (53) having a predetermined temperature and arranged in series or in parallel and separating a DNA complementary to the stationary-phase DNA probe (53).
The above-mentioned gene analysis method comprises a plurality of stationary-phase DNA probes (64 through 66) at least a part of which can be set to a temperature for forming a double strand of DNA to be tested and a reaction eluent containing the same or different kinds of DNA which have been amplified, is introduced into the stationary-phase DNA probes (64 through 66).
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