发明公开
EP1481090A1 METHODS AND REAGENTS FOR DETECTING TARGET BINDING BY NUCLEIC ACID LIGANDS
审中-公开
方法和试剂进行目标分子的检测同位素标记配体的结合
- 专利标题： METHODS AND REAGENTS FOR DETECTING TARGET BINDING BY NUCLEIC ACID LIGANDS
- 专利标题（中）： 方法和试剂进行目标分子的检测同位素标记配体的结合
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申请号： EP03713423.6申请日： 2003-02-10
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公开(公告)号： EP1481090A1公开(公告)日： 2004-12-01
- 发明人: GOLD, Larry , ZICHI, Dominic, A. , SMITH, Jonathan, Drew , SCHNEIDER, Daniel, J. , GREEF, Chad
- 申请人： SomaLogic, Inc.
- 申请人地址： 1775 38th Street Boulder, CO 80301 US
- 专利权人： SomaLogic, Inc.
- 当前专利权人： SomaLogic, Inc.
- 当前专利权人地址： 1775 38th Street Boulder, CO 80301 US
- 代理机构： Clegg, Richard Ian
- 优先权： US357297P 20020215; US114187 20020401; US398666P 20020726; US400759P 20020802
- 国际公布： WO2003070984 20030828
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12P19/34 ; C12M1/34 ; C07H21/02 ; C07H21/04 ; C07H19/00
摘要：
The present invention provides novel methods and reagents for detecting the binding of protein targets to nucleic acid ligands. Using Universal Protein Stains (UPS), proteins bound by nucleic acid ligands may be labeled with a detectable moiety. The methods and reagents are particularly useful for the detection of protein targets bound to multiplexed arrays of nucleic acid ligands. The present invention also provides novel methods for the multiplexed evaluation of photocrosslinking nucleic acid ligands. The methods allow one simultaneously to: (1) evaluate the performance (dynamic range) of a plurality of photocrosslinking nucleic acid ligands; and (2) assess the specificity of each photocrosslinking nucleic acid ligand for its cognate target protein. Photocrosslinking nucleic acid ligands with the most desirable properties can then be selected for use in diagnostic and prognostic medical assays. The present invention also provides a photocrosslinking nucleic acid ligand that binds specifically to HIV gp120MN.
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