发明授权
- 专利标题: PROCESS FOR PRODUCING HETEROLOGOUS PROTEIN IN E. COLI
- 专利标题(中): PROCESS FOR在大肠杆菌中产生异源蛋白质
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申请号: EP04713206.3申请日: 2004-02-20
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公开(公告)号: EP1602720B1公开(公告)日: 2010-11-03
- 发明人: KOYANAGI, Satoshi, Chemo-sero-therap. res.inst. , SUGAWARA, Keishin, Chemo-sero-therap. res.inst. , MIYATSU, Yoshinobu, Chemo-sero-therap. res. inst. , MURAKAMI, Toshio, Chemo-sero-therap. research inst , MAEDA, Toshihiro, Chemo-sero-therapeut. res.inst. , MIZOKAMI, Hiroshi, Chemo-sero-therap. res.inst.
- 申请人: Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
- 申请人地址: 6-1, Okubo 1-chome Kumamoto-shi, Kumamoto 860-8568 JP
- 专利权人: Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
- 当前专利权人: Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
- 当前专利权人地址: 6-1, Okubo 1-chome Kumamoto-shi, Kumamoto 860-8568 JP
- 代理机构: Vossius & Partner
- 优先权: JP2003058992 20030305
- 国际公布: WO2004078965 20040916
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; C12P21/02 ; C07K14/435
摘要:
It is intended to provide a foreign protein free from any expression inducer and a process for producing the same. Namely, a process for producing a foreign protein involving the step of proliferating a recombinant Escherichia coli, which expresses the foreign protein under the control by a promoter capable of inducing the expression due to the temperature shift, by arbitrarily culturing at a low temperature and then culturing the strain at a high temperature in the absence of an expression inducer to thereby allow the expression of the foreign protein, or the step of culturing the above Escherichia coli strain at a high temperature and thus effecting the proliferation of the cells and the expression of the foreign protein at the same time; and a foreign protein free from any expression inducer which is obtained by this process. Particular examples of such foreign proteins include main tick allergens, Actinogacillus pleuropneumoniae-origin secretory macrophage toxin and a protective antigen against Erysipelothrix rhusiopathiae-infection.
公开/授权文献
- EP1602720A1 PROCESS FOR PRODUCING FOREIGN PROTEIN IN ESCHERICHIA COLI 公开/授权日:2005-12-07
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