发明公开
EP1736554A3 Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
失效
通过Ligasebestimmung的组合和聚合酶链反应确定所述Nukleisäuresequenz差异
- 专利标题： Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
- 专利标题（中）： 通过Ligasebestimmung的组合和聚合酶链反应确定所述Nukleisäuresequenz差异
-
申请号： EP06011667.0申请日： 1997-05-27
-
公开(公告)号： EP1736554A3公开(公告)日： 2007-02-21
- 发明人: Belgrader, Phillip , Barany, Francis , Lubin, Matthew , Barany, George , Hammer, Robert P.
- 申请人： CORNELL RESEARCH FOUNDATION, INC. , Belgrader, Phillip
- 申请人地址： 20 Thornwood Drive, Suite 105 Ithaca, NY 14850 US
- 专利权人： CORNELL RESEARCH FOUNDATION, INC.,Belgrader, Phillip
- 当前专利权人： CORNELL RESEARCH FOUNDATION, INC.,Belgrader, Phillip
- 当前专利权人地址： 20 Thornwood Drive, Suite 105 Ithaca, NY 14850 US
- 代理机构： Miles, John Stephen
- 优先权： US18532P 19960529
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.
公开/授权文献
信息查询
