发明公开
- 专利标题: GEGEN DI- UND/ODER MULTIMERISIERUNG STABILISIERTE ALPHA-AMYLASE-VARIANTEN, VERFAHREN ZU DEREN HERSTELLUNG SOWIE WASCH- UND REINIGUNGSMITTEL MIT DIESEN ALPHA-AMYLASE-VARIANTEN
- 专利标题(英): Alpha-amylase variants stabilized against dimerization and/or multimerization, method for the production thereof, and detergents and cleansers containing these alpha-amylase variants
- 专利标题(中): 反对二和/或多聚稳定化的α-淀粉酶变,生产和洗涤方法与这些α淀粉酶的变体清洁
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申请号: EP05788794.5申请日: 2005-09-22
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公开(公告)号: EP1794292A2公开(公告)日: 2007-06-13
- 发明人: BESSLER, Cornelius , WIELAND, Susanne , MAURER, Karl-Heinz
- 申请人: HENKEL KOMMANDITGESELLSCHAFT AUF AKTIEN
- 申请人地址: Henkelstrasse 67 40589 Düsseldorf DE
- 专利权人: HENKEL KOMMANDITGESELLSCHAFT AUF AKTIEN
- 当前专利权人: HENKEL KOMMANDITGESELLSCHAFT AUF AKTIEN
- 当前专利权人地址: Henkelstrasse 67 40589 Düsseldorf DE
- 优先权: DE102004047776 20041001
- 国际公布: WO2006037483 20060413
- 主分类号: C12N9/28
- IPC分类号: C12N9/28 ; C11D3/386
摘要:
The invention relates to α-amylase variants, which are stabilized with regard to dimerization and/or multimerization by the point mutagenesis of positively polarized or charged or neutral surface amino acids into negatively polarized or charged amino acids. The invention also relates to methods for increasing the stability of an α-amylase with regard to a dimerization and/or multimerization induced by electrostatic interactions, whereby at least one amino acid radical of the starting molecule, which is located on the surface of the molecule and which exerts a neutral or positively polar or charged contribution to the electrostatic potential of the molecule, is replaced by a stronger negatively polar or negatively charged amino acid radical. The α-amylase variants obtained in this manner exhibit an improved processivity and are suited for numerous technical areas of application, particularly for use as active ingredients in detergents and cleansers.
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