Confining structure (5) to confine the queen bee of a colony of bees within a beehive comprising a confining body (16), a reception chamber provided in said containing body to receive said queen bee, at least one introduction opening (16) provided on said containing body and communicating with said chamber for introducing said queen bee in said chamber, occluding means capable of being coupled/uncoupled to/from said containing body to occlude said opening, and a plurality of feeding slots (25) provided on said containing body and communicating with said chamber so as to allow said queen bee to be fed on the part of said colony of bees, and suspending means (26) associated with said containing body to suspend said containing body within said beehive nest, so as to maintain the proximity of said queen bee to said colony of bees during the confining period of said queen bee. The structure solves the drawbacks and problems found in the prior art, as it allows providing an ideal arrangement of the queen bee with respect to its colony of bees over a sufficiently prolonged period of confinement.