The invention relates to a cell culture device. This cell culture device (100), intended for receiving cells with their culture medium, is characterised in that it contains at least one cell-supporting layer of porous material, this layer being obtained by dehydration of an aqueous gel which is poured directly either into said well bottom (110-111), or into the bottom of a cradle or insert (150) of a size which is adapted for being insertable into the volume of the well. The invention enables a culture to be made which can be automated.