The method according to the invention consists of generating an electrochemical signature of the analyzed substance(s), obtained by carrying out a numerical pseudo-titration and to express the result of the measurements in antioxidant power units. It involves the processing of a current-potential response of the oxidation of the analyzed substance(s) by a predefined mathematical dimensionless function representing a virtual and ideal oxidizing agent. The method is useful for the identification and/or detection and/or titration of antioxidant substances in the tested material, including directly on wet biological tissues.