发明授权
- 专利标题： HLA-B GENOTYPING METHOD AND KIT BASED ON REAL-TIME PCR
- 专利标题（中）： HLA-B基因分型和-KIT实时PCR BASED
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申请号： EP06841799.7申请日： 2006-12-12
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公开(公告)号： EP1964929B1公开(公告)日： 2012-02-15
- 发明人: JUAN OTERO, Manuel , CASAMITJANA PONCES, Natàlia , PALOU RIVERA, Eduardo , PUJOL BORRELL, Ricardo , FANER CANET, María Rosa
- 申请人： Universitat Autònoma de Barcelona , Banc de Sang i Teixits
- 申请人地址： Àrea l + D- Edifici A-Campus Universitari s/n 08193 Bellaterra ES
- 专利权人： Universitat Autònoma de Barcelona,Banc de Sang i Teixits
- 当前专利权人： Universitat Autònoma de Barcelona,Banc de Sang i Teixits
- 当前专利权人地址： Àrea l + D- Edifici A-Campus Universitari s/n 08193 Bellaterra ES
- 代理机构： ZBM Patents
- 优先权： ES200503110 20051213
- 国际公布： WO2007068782 20070621
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; G01N33/58
摘要：
The invention relates to a HLA-B genotyping method and kit based on real-time PCR with specific probes and primers which enable a high level of subtyping of the complete HLA-B locus. The invention offers the following main advantages, namely: increased speed (65 minutes including interpretation), ease of automation since only 18 tubes are required in order to obtain a good resolution level (typing of 300 groups), a reduction in the total cost per test owing to the ease of automation and the simplicity thereof, a surprisingly high degree of allelic definition, and a reduction in the sample contamination risks such that the amplified products always appear in the tubes and no post-PCR step is necessary.
公开/授权文献
- EP1964929A1 HLA-B GENOTYPING METHOD AND KIT BASED ON REAL-TIME PCR 公开/授权日：2008-09-03
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