发明公开
EP2014766A1 METHOD FOR DIFFERENTIATION INDUCTION OF MYOCARDIAL CELL FROM PLURIPOTENT STEM CELL
审中-公开
Methode zur Induktion der Differenzierung von Myokardzellen aus pluripotenten Stammzellen
- 专利标题： METHOD FOR DIFFERENTIATION INDUCTION OF MYOCARDIAL CELL FROM PLURIPOTENT STEM CELL
- 专利标题（中）： Methode zur Induktion der Differenzierung von Myokardzellen aus pluripotenten Stammzellen
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申请号： EP07742677.3申请日： 2007-04-27
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公开(公告)号： EP2014766A1公开(公告)日： 2009-01-14
- 发明人: KOSHIMIZU, Uichi , TANAKA, Tomofumi , KAWASHIMA, Kayoko , KADOKURA, Michinori
- 申请人： Asubio Pharma Co., Ltd.
- 申请人地址： 9-11 Akasaka 2-chome Minato-ku Tokyo 107-8541 JP
- 专利权人： Asubio Pharma Co., Ltd.
- 当前专利权人： Asubio Pharma Co., Ltd.
- 当前专利权人地址： 9-11 Akasaka 2-chome Minato-ku Tokyo 107-8541 JP
- 代理机构： Vossius & Partner
- 优先权： JP2006125148 20060428; JP2007019531 20070130
- 国际公布： WO2007126077 20071108
- 主分类号： C12N5/00
- IPC分类号： C12N5/00 ; C07K14/47 ; C12N5/06 ; A61L27/00 ; C12N15/09
摘要：
The present invention provides a method for inducing differentiation of cardiomyocytes efficiently and selectively from stem cells.
A method for inducing differentiation of cardiomyocytes from pluripotent stem cells, which comprises: (i) culturing the pluripotent stem cells in a culture medium containing no substance that promotes activation of the canonical Wnt signaling pathway during the time period between initiation of differentiation induction and 24 hours before the period of elevated canonical Wnt gene expression; and then (ii) culturing the pluripotent stem cells in a culture medium containing a substance that promotes activation of the canonical Wnt signaling pathway during a time period of 24 to 96 hours, starting from 24 to 0 hours before the period of elevated canonical Wnt gene expression.
A method for inducing differentiation of cardiomyocytes from pluripotent stem cells, which comprises: (i) culturing the pluripotent stem cells in a culture medium containing no substance that promotes activation of the canonical Wnt signaling pathway during the time period between initiation of differentiation induction and 24 hours before the period of elevated canonical Wnt gene expression; and then (ii) culturing the pluripotent stem cells in a culture medium containing a substance that promotes activation of the canonical Wnt signaling pathway during a time period of 24 to 96 hours, starting from 24 to 0 hours before the period of elevated canonical Wnt gene expression.
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