发明公开
EP2045264A3 Novel pH- and anion concentration-responsive GFP mutant, a chimeric protein comprising such a mutant and a method for the combined assaying of the pH and anion concentration
有权
新，响应于pH和阴离子浓度GFP突变体，与pH和阴离子的组合分析这些突变体和方法嵌合蛋白
- 专利标题： Novel pH- and anion concentration-responsive GFP mutant, a chimeric protein comprising such a mutant and a method for the combined assaying of the pH and anion concentration
- 专利标题（中）： 新，响应于pH和阴离子浓度GFP突变体，与pH和阴离子的组合分析这些突变体和方法嵌合蛋白
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申请号： EP08165522.7申请日： 2008-09-30
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公开(公告)号： EP2045264A3公开(公告)日： 2009-04-29
- 发明人: Arosio, Daniele , Beltram, Fabio , Ricci, Fernanda , Marchetti, Laura
- 申请人： Consiglio Nazionale delle Ricerche - INFM Istituto Nazionale per la Fisica della Materia
- 申请人地址： Corso F. Perrone, 24 16152 Genova IT
- 专利权人： Consiglio Nazionale delle Ricerche - INFM Istituto Nazionale per la Fisica della Materia
- 当前专利权人： Consiglio Nazionale delle Ricerche - INFM Istituto Nazionale per la Fisica della Materia
- 当前专利权人地址： Corso F. Perrone, 24 16152 Genova IT
- 代理机构： Comoglio, Elena
- 优先权： ITTO20070687 20071001
- 主分类号： C07K14/435
- IPC分类号： C07K14/435 ; G01N33/52 ; G01N33/84
摘要：
A mutant of the GFP fluorescent protein, designated as D3GFP, is described, the fluorescence of which is sensitive to both the pH and anion concentration. A chimeric protein comprising a first protein, P1, and a second protein, P2, is further described, wherein the P1 protein is a fluorescent protein sensitive to the pH and anion concentration - such as the D3GFP mutant - and the P2 protein is a fluorescent protein, the fluorescence of which is independent from both the pH and anion concentration. In addition, P2 is selected so that there is no substantial overlapping of the P1 and P2 emission and excitation fluorescence spectra. A method is also described for the combined measurement of the pH and anion concentration (for instance the intracellular chlorine ions) by using the said chimeric protein.
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