发明公开
EP2298935A1 Methods for detection of a target nucleic acid sequence
审中-公开
Verfahren zur Detektion einerZielnukleinsäuresequenz
- 专利标题： Methods for detection of a target nucleic acid sequence
- 专利标题（中）： Verfahren zur Detektion einerZielnukleinsäuresequenz
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申请号： EP10179704.1申请日： 2000-10-27
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公开(公告)号： EP2298935A1公开(公告)日： 2011-03-23
- 发明人: Sorge, Joseph, A.
- 申请人： Hologic Inc.
- 申请人地址： 250 Campus Drive Marlborough, MA 01752 US
- 专利权人： Hologic Inc.
- 当前专利权人： Hologic Inc.
- 当前专利权人地址： 250 Campus Drive Marlborough, MA 01752 US
- 代理机构： Tombling, Adrian George
- 优先权： US430692 19991029
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample, where the method includes forming a cleavage structure by incubating a sample containing a target nucleic acid sequence with a nucleic acid polymerase and cleaving the cleavage structure with an FEN nuclease to generate a cleaved nucleic acid fragment. The invention also relates to methods of detecting or measuring a target nucleic acid sequence, where the method includes forming a cleavage structure by incubating a target nucleic acid sequence with a nucleic acid polymerase, cleaving the cleavage structure with an FEN nuclease and detecting or measuring the release of a fragment.
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