- 专利标题： Thermostable biocatalyst combination for nucleoside synthesis
- 专利标题（中）： 生物催化组合
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申请号： EP09382296.3申请日： 2009-12-22
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公开(公告)号： EP2338985A1公开(公告)日： 2011-06-29
- 发明人: Montilla Arevalo, Rafael , Deroncelé Thomas, Víctor Manuel , López Gómez, Cristina , Pascual Gilabert, Marta , Estévez Company, Carlos , Castells Boliart, Josep
- 申请人： Institut Univ. de Ciència i Tecnologia, s.a.
- 申请人地址： C/Álvarez de Castro, 63 08100 Mollet del Vallès, Barcelona ES
- 专利权人： Institut Univ. de Ciència i Tecnologia, s.a.
- 当前专利权人： Institut Univ. de Ciència i Tecnologia, s.a.
- 当前专利权人地址： C/Álvarez de Castro, 63 08100 Mollet del Vallès, Barcelona ES
- 代理机构： Ponti Sales, Adelaida
- 主分类号： C12N9/10
- IPC分类号： C12N9/10 ; C12P19/38
摘要：
The present invention relates to a transglycosylation method for the preparation of natural and synthetic nucleosides using a uridine phosphorylase (PyNPase, E.C. 2.4.2.3), a purine nucleoside phosphorylase (PNPase, E.C. 2.4.2.1), or a combination thereof. These biocatalysts may be used as such, or by means of host cells transformed with vectors comprising recombinant DNA gene derived from hyperthermophilic archaea and encoding for the PyNPase and PNPase enzymes.
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