发明公开
EP2369007A1 Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
失效
通过同时Ligaseerkennungs-和聚合酶链反应的手段的核酸序列的差异检测
- 专利标题： Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
- 专利标题（中）： 通过同时Ligaseerkennungs-和聚合酶链反应的手段的核酸序列的差异检测
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申请号： EP10075461.3申请日： 1997-05-27
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公开(公告)号： EP2369007A1公开(公告)日： 2011-09-28
- 发明人: Belgrader, Phillip , Barany, Francis , Lubin, Matthew , Barany, George , Hammer, Robert P
- 申请人： CORNELL RESEARCH FOUNDATION, INC.
- 申请人地址： 20 Thornwood Drive, Suite 105 Ithaca, NY 14850 US
- 专利权人： CORNELL RESEARCH FOUNDATION, INC.
- 当前专利权人： CORNELL RESEARCH FOUNDATION, INC.
- 当前专利权人地址： 20 Thornwood Drive, Suite 105 Ithaca, NY 14850 US
- 代理机构： Miles, John Stephen
- 优先权： US18532P 19960529
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.
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