- 专利标题： Aldolases, nucleic acids encoding them and methods for making and using them
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申请号： EP11167410.7申请日： 2007-03-07
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公开(公告)号： EP2385108A1公开(公告)日： 2011-11-09
- 发明人: Burke, Ellen , Hicks, Paula M. , Luginbuhl, Peter , Richardson, Toby , Weiner, David P. , Zhao, Lishan
- 申请人： Verenium Corporation
- 申请人地址： Intellectual Property Department P.O. Box 910550 San Diego CA 92191-0550 US
- 专利权人： Verenium Corporation
- 当前专利权人： Verenium Corporation
- 当前专利权人地址： Intellectual Property Department P.O. Box 910550 San Diego CA 92191-0550 US
- 代理机构： Maiwald Patentanwalts GmbH
- 优先权： US780515P 20060307
- 主分类号： C12N9/10
- IPC分类号： C12N9/10 ; C12N15/82 ; C12N9/88
摘要：
This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.
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