发明公开
EP2408476A2 HUMAN IMMUNODEFICIENCY VIRUS (HIV) -NEUTRALIZING ANTIBODIES
有权
DAS HUMANEIMMUNSCHWÄCHEVIRUS（艾滋病毒）中性粒细胞抗体
- 专利标题： HUMAN IMMUNODEFICIENCY VIRUS (HIV) -NEUTRALIZING ANTIBODIES
- 专利标题（中）： DAS HUMANEIMMUNSCHWÄCHEVIRUS（艾滋病毒）中性粒细胞抗体
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申请号： EP10722810.8申请日： 2010-03-17
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公开(公告)号： EP2408476A2公开(公告)日： 2012-01-25
- 发明人: CHAN-HUI, Po-Ying , MITCHAM, Jennifer , OLSEN, Ole , FREY, Steven , MOYLE, Matthew , PHOGAT, Sanjay, K. , BURTON, Dennis, R. , WALKER, Laura, Marjorie , POIGNARD, Pascal, Raymond, Georges , DE JEAN DE ST.MARCEL SIMEK-LEMOS, Melissa, D. , KOPF, Wayne , KAMINSKY, Stephen
- 申请人： Theraclone Sciences, Inc. , The Scripps Research Institute , International AIDS Vaccine Initiative
- 申请人地址： Seattle Life Sciences Building 1124 Columbia Street Suite 300 Seattle, WA 98104 US
- 专利权人： Theraclone Sciences, Inc.,The Scripps Research Institute,International AIDS Vaccine Initiative
- 当前专利权人： Theraclone Sciences, Inc.,The Scripps Research Institute,International AIDS Vaccine Initiative
- 当前专利权人地址： Seattle Life Sciences Building 1124 Columbia Street Suite 300 Seattle, WA 98104 US
- 代理机构： Schüssler, Andrea
- 优先权： US224739P 20090710; US161010P 20090317; US165829P 20090401; US285664P 20091211
- 国际公布： WO2010107939 20100923
- 主分类号： A61K39/118
- IPC分类号： A61K39/118 ; A61K39/39 ; C07K16/10
摘要：
The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies are characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.
公开/授权文献
- EP2408476B1 HUMAN IMMUNODEFICIENCY VIRUS (HIV) -NEUTRALIZING ANTIBODIES 公开/授权日：2017-10-18
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