发明公开
- 专利标题： DIGITAL QUANTIFICATION OF DNA METHYLATION
- 专利标题（中）： DNA甲基化的定量数字
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申请号： EP10762298.7申请日： 2010-04-06
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公开(公告)号： EP2417270A2公开(公告)日： 2012-02-15
- 发明人: VOGELSTEIN, Bert , KINZLER, Kenneth, W. , LI, Meng , DIAZ, Luis , PAPADOPOULOS, Nickolas , MARKOWITZ, Sanford
- 申请人： The Johns Hopkins University , Case Western Reserve University
- 申请人地址： 3400 North Charles Street Baltimore, MD 21218 US
- 专利权人： The Johns Hopkins University,Case Western Reserve University
- 当前专利权人： The Johns Hopkins University,Case Western Reserve University
- 当前专利权人地址： 3400 North Charles Street Baltimore, MD 21218 US
- 代理机构： Tombling, Adrian George
- 优先权： US166970P 20090406
- 国际公布： WO2010118016 20101014
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; C12N15/11 ; G01N33/52
摘要：
Abnormal DNA methylation can be used as a biomarker in cancer patients. For such purposes, it is important to determine precisely the fraction of methylated molecules in an analyzed sample. A technology we term Methyl-BEAMing achieves this goal. Individual bisulfite-treated DNA molecules can be PCR-amplified within aqueous nanocompartments containing beads, resulting in a population of beads each containing thousands of copies of the template molecule. After hybridization with probes specific for methylated sequences, the beads can be analyzed by flow cytometry. This approach enables detection and enumeration of one methylated molecule in a population of ~5000 unmethylated molecules. Methyl-BEAMing provides digital quantification of rare methylation events and is generally applicable to the assessment of methylated genes in clinical samples.
公开/授权文献
- EP2417270B1 DIGITAL QUANTIFICATION OF DNA METHYLATION 公开/授权日：2017-09-20
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