发明公开
EP2460820A3 PCV2 immunogenic compositions and methods of producing such compositions
审中-公开
PCV2免疫原性组合物和生产这种组合物的方法
- 专利标题： PCV2 immunogenic compositions and methods of producing such compositions
- 专利标题（中）： PCV2免疫原性组合物和生产这种组合物的方法
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申请号： EP12155382.0申请日： 2005-12-29
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公开(公告)号： EP2460820A3公开(公告)日： 2013-01-09
- 发明人: Eichmeyer, Mark , Nitzel, Greg , Schaeffer, Merrill
- 申请人： Boehringer Ingelheim Vetmedica, Inc.
- 申请人地址： 2621 North Belt Highway St. Joseph, MO 64506-2002 US
- 专利权人： Boehringer Ingelheim Vetmedica, Inc.
- 当前专利权人： Boehringer Ingelheim Vetmedica, Inc.
- 当前专利权人地址： 2621 North Belt Highway St. Joseph, MO 64506-2002 US
- 代理机构： HOFFMANN EITLE
- 优先权： US640510P 20041230; US34797 20050113; US319975 20051229
- 主分类号： C07K14/01
- IPC分类号： C07K14/01 ; A61K39/12 ; C12N7/04 ; C12Q1/68 ; A61K39/295
摘要：
An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
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