发明授权
EP2460888B1 METHOD AND KIT FOR MEASUREMENT OF DEHYDROGENASE OR SUBSTRATE FOR THE DEHYDROGENASE
有权
用于测定脱氢酶或脱氢酶的底物的方法和试剂盒
- 专利标题： METHOD AND KIT FOR MEASUREMENT OF DEHYDROGENASE OR SUBSTRATE FOR THE DEHYDROGENASE
- 专利标题（中）： 用于测定脱氢酶或脱氢酶的底物的方法和试剂盒
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申请号： EP10804211.0申请日： 2010-06-21
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公开(公告)号： EP2460888B1公开(公告)日： 2018-05-09
- 发明人: NODA Kenta , SATO Yoshiro , KOJIMA Ryo
- 申请人： Nitto Boseki CO., LTD.
- 申请人地址： 1, Aza Higashi Gonome Fukushima-shi Fukushima 960-8161 JP
- 专利权人： Nitto Boseki CO., LTD.
- 当前专利权人： Nitto Boseki CO., LTD.
- 当前专利权人地址： 1, Aza Higashi Gonome Fukushima-shi Fukushima 960-8161 JP
- 代理机构： Hoffmann Eitle
- 优先权： JP2009175379 20090728
- 国际公布： WO2011013464 20110203
- 主分类号： C12Q1/32
- IPC分类号： C12Q1/32 ; C12Q1/54 ; C12Q1/58
摘要：
Disclosed is a method for measuring a dehydrogenase or a substrate for the dehydrogenase contained in a sample, wherein both thio-NAD or thio-NADP and NAD or NADP are used as coenzymes. The method enables the measurement on the basis of absorbance values obtained in a visible region, and also enables the accurate measurement of a dehydrogenase or a substrate for the dehydrogenase which is contained in a sample at a high concentration.
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