发明授权
- 专利标题： NOVEL PROTEIN HAVING B-GLUCOSIDASE ACTIVITY, AND USE THEREOF
- 专利标题（中）： 具有β-葡糖苷酶活性的新蛋白质及其用途
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申请号： EP10809957.3申请日： 2010-08-17
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公开(公告)号： EP2468859B1公开(公告)日： 2018-02-21
- 发明人: YOKOYAMA, Fumikazu , YOKOYAMA, Kengo , MAZUKA, Nobuko
- 申请人： Meiji Seika Pharma Co., Ltd.
- 申请人地址： 4-16, Kyobashi 2-chome Chuo-ku Tokyo 104-8002 JP
- 专利权人： Meiji Seika Pharma Co., Ltd.
- 当前专利权人： Meiji Seika Pharma Co., Ltd.
- 当前专利权人地址： 4-16, Kyobashi 2-chome Chuo-ku Tokyo 104-8002 JP
- 代理机构： Cohausz & Florack
- 优先权： JP2009190840 20090820
- 国际公布： WO2011021616 20110224
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C12N1/15 ; C12N1/19 ; C12N1/21 ; C12N5/10 ; C12N9/42 ; C12P19/14 ; C12P21/02 ; D21C5/02 ; D21H17/22 ; D21C5/00 ; D21H17/00 ; D21H21/10 ; D06M15/15 ; A23K10/32 ; A23K10/14
摘要：
By combination of hydrophobic chromatography and strongly basic anion-exchange chromatography, a novel, highly hydrophobic ²-glucosidase was successfully identified from Acremonium cellulolyticus. Further, a gene corresponding to the identified ²-glucosidase was isolated. When multiple modifications were introduced into the base sequence of the gene, the gene was successfully expressed in Trichoderma viride at a high level, and the expression product successfully exhibited a high ²-glucosidase activity.
公开/授权文献
- EP2468859A1 NOVEL PROTEIN HAVING B-GLUCOSIDASE ACTIVITY, AND USE THEREOF 公开/授权日：2012-06-27
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