An optical fiber holder that allows high strength fibers to be readily handled with no loss of splicing quality when the optical fiber holder holds a coated optical fiber is provided. An optical fiber holder 1 includes a holder main body 6, and the holder main body 6 has a fiber receiving groove 7 that receives and positions the coated optical fiber exposed out of the optical fiber cord from which a cord jacket is removed at the tip of the optical fiber cord and a cord receiving groove 8 that receives the cord jacket. A cord holding cover 12 and a fiber pressing cover 13 are attached to the holder main body 6 in an openable and closable manner. A cord receiving groove 15 that cooperates with the cord receiving groove 8 to form an insertion through hole having a cross-section being circular and allowing the optical fiber cord to be inserted is formed on the back side of the cord holding cover 12. The diameter of the insertion through hole is slightly greater than that of the cord jacket. The fiber pressing cover 13 presses the coated optical fiber received by the fiber receiving groove 7 against the holder main body 6.