A tissue array production method which enables even roll-shaped tissue pieces having various diameters to be steadily fixed to a substrate block is provided. In the tissue array production method in which roll-shaped tissue pieces 6 formed by rolling sheet-like tissue pieces 8 in the shape of a roll are arranged on a substrate in an array form, the tissue array is produced by placing a holding member 16 which holds a plurality of roll-shaped tissue pieces 6 so that their axis directions are vertically directed in a container 10 into which a melted embedding medium is poured for its accumulation, to hold the respective roll-shaped tissue pieces 6 in the holding member 16; by pouring the embedding medium into the container 10, to form a substrate block 40 constituted so that the plurality of roll-shaped tissue pieces 6 is arranged in the array form; and by slicing the substrate block 40 so that the roll-shaped tissue pieces 6 are ring-shaped, to place the sliced pieces on the substrate.