发明公开
- 专利标题： SOLUTION FOR EXTRACTION OF RNA
- 专利标题（中）： 解RNA提取
-
申请号： EP11818210.4申请日： 2011-08-17
-
公开(公告)号： EP2607482A1公开(公告)日： 2013-06-26
- 发明人: YOSHIMOTO, Makiko , AKIYAMA, Hideo , NOBUMASA, Hitoshi
- 申请人： Toray Industries, Inc.
- 申请人地址： 1-1, Nihonbashi-Muromachi 2-chome Chuo-ku Tokyo 103-8666 JP
- 专利权人： Toray Industries, Inc.
- 当前专利权人： Toray Industries, Inc.
- 当前专利权人地址： 1-1, Nihonbashi-Muromachi 2-chome Chuo-ku Tokyo 103-8666 JP
- 代理机构： Kador & Partner
- 优先权： JP2010183280 20100818
- 国际公布： WO2012023569 20120223
- 主分类号： C12N15/09
- IPC分类号： C12N15/09
摘要：
A solution for extracting substantially pure RNA from a biological sample is disclosed. The solution for extracting RNA from a biological sample containing RNA and at least DNA comprises:
(a) phenol in an amount of more than 50% by volume based on the total amount of the solution;
(b) a polyol in an amount of 3 to 10% by volume based on the total amount of the solution;
(c) a guanidinium salt at a concentration of 0.5 to 2.0 M based on the total amount of the solution;
(d) a thiocyanate at a concentration of 0.1 to 0.5 M based on the total amount of the solution; and
(e) a buffer for maintaining the pH of the solution at 4 to 6.
(a) phenol in an amount of more than 50% by volume based on the total amount of the solution;
(b) a polyol in an amount of 3 to 10% by volume based on the total amount of the solution;
(c) a guanidinium salt at a concentration of 0.5 to 2.0 M based on the total amount of the solution;
(d) a thiocyanate at a concentration of 0.1 to 0.5 M based on the total amount of the solution; and
(e) a buffer for maintaining the pH of the solution at 4 to 6.
公开/授权文献
- EP2607482B1 SOLUTION FOR EXTRACTION OF RNA 公开/授权日：2017-04-05
信息查询
IPC分类:
