发明公开
- 专利标题： PROTEIN PRODUCTION METHOD
- 专利标题（中）： PROTEINHERSTELLUNGSVERFAHREN
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申请号： EP11848424.5申请日： 2011-12-14
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公开(公告)号： EP2653540A1公开(公告)日： 2013-10-23
- 发明人: KAWAKAMI Koichi , KUROKAWA Megumi , YAMAGUCHI Keina , OGAWA Risa , TSUKAHARA Masayoshi , HAYASHI Yoko
- 申请人： Inter-University Research Institute Corporation Research Organization of Information and Systems , Kyowa Hakko Kirin Co., Ltd.
- 申请人地址： 10-3, Midori-cho, Tachikawa-shi, Tokyo 190-0014 JP
- 专利权人： Inter-University Research Institute Corporation Research Organization of Information and Systems,Kyowa Hakko Kirin Co., Ltd.
- 当前专利权人： Inter-University Research Institute Corporation Research Organization of Information and Systems,Kyowa Hakko Kirin Co., Ltd.
- 当前专利权人地址： 10-3, Midori-cho, Tachikawa-shi, Tokyo 190-0014 JP
- 代理机构： Woods, Geoffrey Corlett
- 优先权： JP2010279849 20101215
- 国际公布： WO2012081628 20120621
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C12N5/10 ; C12P21/02 ; C12P21/08
摘要：
The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.
公开/授权文献
- EP2653540B9 PROTEIN PRODUCTION METHOD 公开/授权日：2018-05-16
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