发明公开
EP2717676A1 METHOD FOR THE GENERATION OF A MONOCLONAL PLANT CELL LINE
审中-公开
VERFAHREN ZUR ERZEUGUNG EINER MONOKLONALEN PFLANZENZELLLINIE
- 专利标题： METHOD FOR THE GENERATION OF A MONOCLONAL PLANT CELL LINE
- 专利标题（中）： VERFAHREN ZUR ERZEUGUNG EINER MONOKLONALEN PFLANZENZELLLINIE
-
申请号： EP11729369.6申请日： 2011-06-07
-
公开(公告)号： EP2717676A1公开(公告)日： 2014-04-16
- 发明人: KIRCHHOFF, Janina , SCHILLBERG, Stefan , SCHIERMEYER, Andreas , SCHINKEL, Helga , FISCHER, Rainer
- 申请人： Fraunhofer Gesellschaft zur Förderung der angewandten Forschung E.V.
- 申请人地址： Hansastrasse 27C 80686 München DE
- 专利权人： Fraunhofer Gesellschaft zur Förderung der angewandten Forschung E.V.
- 当前专利权人： Fraunhofer Gesellschaft zur Förderung der angewandten Forschung E.V.
- 当前专利权人地址： Hansastrasse 27C 80686 München DE
- 代理机构： Stürken, Joachim
- 国际公布： WO2012167803 20121213
- 主分类号： A01H4/00
- IPC分类号： A01H4/00
摘要：
The invention provides a method for the generation of a monoclonal plant cell line from a heterologous population of plant cells, comprising the following steps: (a) provision of a heterologous population of plant cells; (b) preparation of protoplasts from said heterologous population of plant cells; (c) separation of single protoplasts by subjecting the preparation of protoplasts to flow cytometric sorting; (d) regeneration of a separated single transformed protoplast until the formation of a microcolony by co-cultivation in the presence of feeder cell material; (e) removal of the microcolony from the feeder cell material and cultivation of the microcolony until the formation of a monoclonal plant cell line.
公开/授权文献
- EP2717676B1 METHOD FOR THE GENERATION OF A MONOCLONAL PLANT CELL LINE 公开/授权日：2019-01-02
信息查询
