发明公开
- 专利标题： METHODS AND COMPOSITIONS FOR TARGETED INTEGRATION IN A PLANT
- 专利标题（中）： 方法和组合物有针对性地引入到植物
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申请号： EP12741436.5申请日： 2012-07-18
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公开(公告)号： EP2739739A1公开(公告)日： 2014-06-11
- 发明人: CIGAN, Andrew Mark , FALCO, Saverio Carl , GAO, Huirong , LASSNER, Michael W. , LI, Zhongsen , LIU, Zhan-Bin , LYZNIK, Leszek A. , PETERSON, David J. , SCELONGE, Christopher Jay
- 申请人： E. I. Du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc.
- 申请人地址： 1007 Market Street Wilmington, Delaware 19899 US
- 专利权人： E. I. Du Pont de Nemours and Company,Pioneer Hi-Bred International, Inc.
- 当前专利权人： E. I. Du Pont de Nemours and Company,Pioneer Hi-Bred International, Inc.
- 当前专利权人地址： 1007 Market Street Wilmington, Delaware 19899 US
- 代理机构： Bentham, Andrew
- 优先权： US201161514565P 20110803
- 国际公布： WO2013019411 20130207
- 主分类号： C12N15/82
- IPC分类号： C12N15/82 ; A01H5/00
摘要：
Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.
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