发明公开
EP2753639A1 POLYPEPTIDES TARGETING GLYCOSYLATED MUC2 PROTEINS, METHODS OF SYNTHESIS, THEIR NUCLEIC ACIDS AND USES THEREOF
有权
ON糖化蛋白MUC2多肽LOOKING，合成，及其核酸和方法及其用途
- 专利标题： POLYPEPTIDES TARGETING GLYCOSYLATED MUC2 PROTEINS, METHODS OF SYNTHESIS, THEIR NUCLEIC ACIDS AND USES THEREOF
- 专利标题（中）： ON糖化蛋白MUC2多肽LOOKING，合成，及其核酸和方法及其用途
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申请号： EP12755887.2申请日： 2012-09-07
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公开(公告)号： EP2753639A1公开(公告)日： 2014-07-16
- 发明人: MARTEYN, Benoit , COIC, Yves-Marie , BALEUX, Françoise , SANSONETTI, Philippe
- 申请人： Institut Pasteur
- 申请人地址： 25-28 Rue du Docteur Roux 75724 Paris Cedex 15 FR
- 专利权人： Institut Pasteur
- 当前专利权人： Institut Pasteur
- 当前专利权人地址： 25-28 Rue du Docteur Roux 75724 Paris Cedex 15 FR
- 代理机构： Desaix, Anne
- 优先权： EP11290403 20110909
- 国际公布： WO2013034749 20130314
- 主分类号： C07K14/195
- IPC分类号： C07K14/195 ; C07K14/335 ; A61K38/16 ; C12N1/15 ; C12N1/21 ; C12N5/10 ; G01N33/68 ; G01N33/58
摘要：
The invention relates to polypeptides, defined through a consensus sequence, having a length from 10 to 80 amino-acid residues, and whose polypeptidic sequence comprises or consists of the consensus sequence P1 (X
a )P3(X
b )P5(X
c )P6(X
d )P7, presenting specific patterns. The polypeptides of the invention target glycosylated Muc2 proteins. The invention also relates to methods of synthesis of such polypeptides, to their nucleic acids and uses thereof. The polypeptidic sequence of the polypeptides of the invention can be part of the N-terminal sequence of a mucus-binding (MUB) domain, especially a mucus-binding (MUB) domain of several species. The invention also relates to chimeric molecule(s) comprising such polypeptides, which are labelled, and vectors, especially plasmids and population of cells or composition comprising polypeptides of the invention. Synthesis methods encompass biotechnological or chemical production. Polypeptides of the invention can be used in staining experiments, as a probe or marker for staining Muc2 protein(s) contained in mucus layer(s), to detect in vitro mucus production or mucus composition in human colon or monitoring any one of the following disease conditions: neoplasic disease(s), including mucinous carcinoma(s), gastric cancer(s) or colorectal cancer(s), especially colon cancer(s), cystic fibrosis, intestine inflammatory disease(s) such as inflammatory bowel disease (IBD) and ulcerative colitis. The invention also relates to a method for manufacturing a medicament. In a particular embodiment, use of polypeptides of the invention can be made for marking neutrophiles.
a )P3(X
b )P5(X
c )P6(X
d )P7, presenting specific patterns. The polypeptides of the invention target glycosylated Muc2 proteins. The invention also relates to methods of synthesis of such polypeptides, to their nucleic acids and uses thereof. The polypeptidic sequence of the polypeptides of the invention can be part of the N-terminal sequence of a mucus-binding (MUB) domain, especially a mucus-binding (MUB) domain of several species. The invention also relates to chimeric molecule(s) comprising such polypeptides, which are labelled, and vectors, especially plasmids and population of cells or composition comprising polypeptides of the invention. Synthesis methods encompass biotechnological or chemical production. Polypeptides of the invention can be used in staining experiments, as a probe or marker for staining Muc2 protein(s) contained in mucus layer(s), to detect in vitro mucus production or mucus composition in human colon or monitoring any one of the following disease conditions: neoplasic disease(s), including mucinous carcinoma(s), gastric cancer(s) or colorectal cancer(s), especially colon cancer(s), cystic fibrosis, intestine inflammatory disease(s) such as inflammatory bowel disease (IBD) and ulcerative colitis. The invention also relates to a method for manufacturing a medicament. In a particular embodiment, use of polypeptides of the invention can be made for marking neutrophiles.
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