The present invention relates to a structure for tissue regeneration and to a production method therefor. The structure for tissue regeneration according to the present invention allows autologous tissue to be used, makes stable transplantation possible and can be produced without additional materials such as biopolymers. Also, it can be produced to a diameter of any desired size from a number of microns to a number of centimeters and can easily be produced in volume, and it contains large amounts of the extracellular matrix GAG, collagen and the like and can be used in tissue regeneration or filling in various fields including dermatology, plastic surgery, dentistry, surgery, orthopaedic surgery, urology, otolaryngology or in obstetrics and gynaecology.