- 专利标题: PEPTIDE HAVING AFFINITY FOR SILICON NITRIDE (SI3N4), AND USE THEREFOR
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申请号: EP13749185.8申请日: 2013-02-12
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公开(公告)号: EP2816110B1公开(公告)日: 2018-11-28
- 发明人: KUMADA, Yoichi , KISHIMOTO, Michimasa , OTSUKA, Takeru
- 申请人: National University Corporation Kyoto Institute of Technology
- 申请人地址: 1 Matsugasaki-Hashikami-cho Sakyo-ku Kyoto-shi, Kyoto 606-8585 JP
- 专利权人: National University Corporation Kyoto Institute of Technology
- 当前专利权人: National University Corporation Kyoto Institute of Technology
- 当前专利权人地址: 1 Matsugasaki-Hashikami-cho Sakyo-ku Kyoto-shi, Kyoto 606-8585 JP
- 代理机构: Patentanwälte Gierlich & Pischitzis Partnerschaft mbB
- 优先权: JP2012028681 20120213
- 国际公布: WO2013122061 20130822
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; C07K14/245 ; C07K17/14 ; C07K19/00 ; C12N1/15 ; C12N1/19 ; C12N1/21 ; C12N5/10 ; C12N11/14 ; C07K7/06 ; C07K7/08 ; C12N9/04 ; C12N15/62
摘要:
The purpose of the present invention is to provide: a peptide having an affinity for silicon nitride; a polynucleotide encoding the peptide; an expression vector for expressing the peptide having an affinity for silicon nitride; an expression vector for expressing a peptide fusion protein that comprises the peptide having an affinity for silicon nitride and a target protein; a transformant obtained by introducing the expression vector into a host cell; a peptide fusion protein obtained from the transformant; a silicon nitride substrate to which a peptide having an affinity for silicon nitride has been bonded; a method for immobilizing a target protein to a silicon nitride substrate; a composition for immobilizing a target protein to a silicon nitride substrate, the composition comprising a peptide having an affinity for silicon nitride; and a linker for immobilizing a target protein to a silicon nitride substrate, the linker comprising a peptide having an affinity for silicon nitride. The invention involves a peptide having an affinity for silicon nitride, the peptide comprising (1-1) a peptide having the amino acid sequence of one of SEQ ID NOS: 1, 2 and 23 to 27, (1-2) a peptide that has an affinity for silicon nitride and comprises an amino acid sequence obtained by deleting, adding, and/or substituting one or more amino acids in one of the amino acid sequences indicated in (1-1), or a fragment of one of the peptides.
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