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发明公开
EP2821498A1 CELL DIFFERENTIATION ASSAY METHOD, CELL ISOLATION METHOD, METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS, AND METHOD FOR PRODUCING DIFFERENTIATED CELLS 审中-公开
细胞分化测试程序方法隔离牢房，方法生成诱导的多能干细胞及其制造方法的分化细胞

CELL DIFFERENTIATION ASSAY METHOD, CELL ISOLATION METHOD, METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS, AND METHOD FOR PRODUCING DIFFERENTIATED CELLS
摘要：
Provided are a method for accurately evaluating the differentiation status of stem cells by selectively staining only stem cells in an undifferentiated state, and a method for positively isolating only stem cells in an undifferentiated state. Specifically provided is a method for determining differentiation of a cell comprising a step of contacting a test cell with a probe comprising protein (A) or (B) below and a step of detecting the presence of binding of the probe to the test cell. The method for determining differentiation of a cell is capable of detecting the presence or absence of an undifferentiated stem cell in test cells by using a probe that specifically reacts with undifferentiated stem cells and detecting the presence of bonding to the test cell. (A) A protein comprising an amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of "Fucα1-2Galβ1-3GlcNAc" and/or "Fucα1-2Galβ1-3GalNAc;" and (B) a protein comprising an amino acid sequence showing 80% or more similarity to the amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of "Fucα1-2Galβ1-3GlcNAc" and/or "Fucα1-2Galβ1-3GalNAc."
摘要（中）：

提供了用于准确的方法设置由染色仅选择性干细胞在未分化的状态的状态评价干细胞的分化状态，和用于积极地只分离干细胞在未分化的方法。 特别提供了一种用于电池，其包括使测试细胞与测试包括蛋白（A）或以下（B）和检测的到测试单元中的探针的结合的存在的步骤的一个步骤的确定性采矿分化的方法。 用于小区的确定性采矿分化的方法，能够通过使用探针检测中测试细胞的未分化的干细胞的存在或不存在的特异性没有反应与未分化的干细胞和检测结合的存在下测试电池。 （A）一种蛋白，其包含在SEQ ID NO：1所示的氨基酸序列：1和认识的 “±Fuc的1-2Gal²1-3GlcNAc” 和/或糖链结构 “±Fuc的1-2Gal²1-3GalNAc;” 和（b）包含于氨基酸序列的蛋白示出80％或更高与SEQ ID NO：2所示的氨基酸序列：1和认识到“±Fuc的1-2Gal²1-3GlcNAc”和/或“岩藻糖的糖链结构 ±1-2Gal²1-3GalNAc“。

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