An air intake system (30, 130) for a work vehicle (10) may include a filter assembly (32, 132) having a pre-cleaner (36, 136) and an air filter (38, 138). The filter assembly (32, 132) may define at least one outlet port (46, 146). The air intake system (30, 130) may also include an air valve (72, 172) movable between an opened position and a closed position and a blower (64, 164) coupled to the outlet port (46, 146) via a conduit (58, 158). The blower (64, 164) may be configured to create a vacuum within the conduit (58 , 158) such that particulates are removed from the filter assembly (32, 132) through the outlet port (46, 146). When the air valve (72, 172) is in the opened position, an airflow may be directed through the filter assembly (32, 132) in an intake direction from the pre-cleaner (36, 136) through the air filter (38, 138) and, when the air valve (72, 172) is in the closed position, the airflow may be directed through the air filter (38, 138) in a reverse direction.