[Problem] To provide a cell culture device capable of controlling the three-dimensional structure of cultured cell aggregates.
[Solution] Provided is a cell culture device 1 having a hydrogel layer 12 formed into a pattern on a substrate 11 and a layer of cell culture part 13 formed on the substrate to cover the hydrogel layer 12. The surface of the cell culture part functions as the plane 14 for seeding a first cell species. The hydrogel layer 12 is impregnated with a cell growth factor, and has a thickness which enables release of the cell growth factor from the hydrogel layer 12 into the cell culture part 13 throughout the cell culture period.