A bio-chamber and a method of making organic fertilizer from poultry manure. The bio-chamber has a chamber body that defines a receiving chamber and an aeration chamber. The aeration chamber underlies the receiving chamber and is divided therefrom by a partition. The partition has a plurality of holes formed therein. An oxygen probe and a temperature probe are positioned inside the receiving chamber. An air blower is connected to the aeration chamber and is operable to blow air therein. The method comprises: mixing the poultry manure with a carbon carrier; loading the mixture into the receiving chamber; monitoring the temperature and the oxygen concentration of the mixture; aerating the mixture; and regulating the temperature and the oxygen concentration by adjusting the operation of the air blower. The temperature and oxygen concentration are regulated to provide conditions that permit aerobic bacteria to propagate and biodegrade the mixture.