- 专利标题： HEAT STABLE KERATINASE AND USE THEREOF
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申请号： EP14831663.1申请日： 2014-07-17
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公开(公告)号： EP3027746B1公开(公告)日： 2018-10-17
- 发明人: HO, Meng-Chiao , WU, Shih-Hsiung , WU, Wan-Ling
- 申请人： Academia Sinica
- 申请人地址： 128, Academia Road, Section 2 Nankang Taipei 11529 TW
- 专利权人： Academia Sinica
- 当前专利权人： Academia Sinica
- 当前专利权人地址： 128, Academia Road, Section 2 Nankang Taipei 11529 TW
- 代理机构： Lang, Christian
- 优先权： US201361859720P 20130729
- 国际公布： WO2015017137 20150205
- 主分类号： C12N9/50
- IPC分类号： C12N9/50
摘要：
A fusion gene encoding M. taiwanensis WR-220 keratinase is disclosed. The fusion comprises: (a) a first DNA sequence encoding a protein secretion signal peptide, located at the N-terminus of the fusion gene; (b) a second DNA sequence encoding an inhibitory domain of M. taiwanensis WR-220 keratinase, linked in translation frame with the first DNA sequence; and (c) a third DNA sequence encoding a catalytic domain of M. taiwanensis WR-220 keratinase, linked in translation frame with the second DNA sequence, wherein the fusion gene is a non-naturally occurring chimeric DNA. Also disclosed are a method for preparation of the catalytic domain of M. taiwanensis WR-220 keratinase, and use of the M. taiwanensis WR-220 keratinase.
公开/授权文献
- EP3027746A1 HEAT STABLE KERATINASE AND USE THEREOF 公开/授权日：2016-06-08
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