A printing system includes a plurality of imaging systems for capturing images of a receiver medium. An illumination system illuminates the receiver medium with an illumination pattern, thereby providing a reflected illumination pattern and a transmitted illumination pattern. A first imaging system is positioned to capture a first image of a first side of the receiver medium including the reflected illumination pattern, and a second imaging system is positioned to capture a second image of an opposing second side of the receiver medium including the transmitted illumination pattern. The first and second images are analyzed to determine a relative position of the reflected illumination pattern in the first image and the transmitted illumination pattern in the second image. Imaging system alignment parameters for use in aligning images captured with the first and second imaging systems are determined responsive to the determined relative position.