发明公开
EP3091080A1 PROCESS FOR PRODUCING ACYL AMINO ACIDS EMPLOYING LIPASES
审中-公开
VERFAHREN ZUR HERSTELLUNG VONACYLAMINOSÄURENUNTER VERWENDUNG VON LIPASEN
- 专利标题： PROCESS FOR PRODUCING ACYL AMINO ACIDS EMPLOYING LIPASES
- 专利标题（中）： VERFAHREN ZUR HERSTELLUNG VONACYLAMINOSÄURENUNTER VERWENDUNG VON LIPASEN
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申请号： EP15166355.6申请日： 2015-05-05
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公开(公告)号： EP3091080A1公开(公告)日： 2016-11-09
- 发明人: Grammann, Katrin , Hennemann, Hans-Georg , Decker, Nicole , Kirchner, Nicole , Reinecke, Liv
- 申请人： Evonik Degussa GmbH
- 申请人地址： Rellinghauser Straße 1- 11 45128 Essen DE
- 专利权人： Evonik Degussa GmbH
- 当前专利权人： Evonik Degussa GmbH
- 当前专利权人地址： Rellinghauser Straße 1- 11 45128 Essen DE
- 主分类号： C12P13/04
- IPC分类号： C12P13/04 ; C12N9/10 ; C12N9/20 ; C12N9/00
摘要：
There is provided a microbial cell for producing at least one acyl amino acid, wherein the cell is genetically modified to comprise:
- a first genetic mutation that increases the expression relative to a wild type cell of at least one lipase (EC 3.1.1) (E 1 ) capable of hydrolysing at least one glyceride to at least one fatty acid; and
- a second and a third genetic mutation that increases the expression relative to a wild type cell of:
(i) an amino acid-N-acyl-transferase (EC 2.3.2) (E 2 ), and
(ii) an acyl-CoA synthetase (EC 6.2.1.1) (E 3 ) respectively
that enables the cell to convert the fatty acid to at least one acyl amino acid and wherein the cell has a reduced fatty acid degradation capacity.
- a first genetic mutation that increases the expression relative to a wild type cell of at least one lipase (EC 3.1.1) (E 1 ) capable of hydrolysing at least one glyceride to at least one fatty acid; and
- a second and a third genetic mutation that increases the expression relative to a wild type cell of:
(i) an amino acid-N-acyl-transferase (EC 2.3.2) (E 2 ), and
(ii) an acyl-CoA synthetase (EC 6.2.1.1) (E 3 ) respectively
that enables the cell to convert the fatty acid to at least one acyl amino acid and wherein the cell has a reduced fatty acid degradation capacity.
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