发明公开
- 专利标题: PSEUDOTYPED LENTIVIRAL VECTORS
- 专利标题(中): 假慢病毒载体
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申请号: EP15700128.0申请日: 2015-01-09
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公开(公告)号: EP3092306A1公开(公告)日: 2016-11-16
- 发明人: ANASTASOV, Natasa , HÖFIG, Ines , THIRION, Christian
- 申请人: Sirion Biotech GmbH , Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)
- 申请人地址: Am Klopferspitz 19 82152 Planegg/Martinsried DE
- 专利权人: Sirion Biotech GmbH,Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)
- 当前专利权人: Sirion Biotech GmbH,Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)
- 当前专利权人地址: Am Klopferspitz 19 82152 Planegg/Martinsried DE
- 代理机构: Vossius & Partner Patentanwälte Rechtsanwälte mbB
- 优先权: EP14150846 20140110
- 国际公布: WO2015104376 20150716
- 主分类号: C12N7/00
- IPC分类号: C12N7/00 ; C12N15/867 ; C07K14/145
摘要:
The present invention relates to a nucleic acid molecule comprising or consisting of a nucleic acid sequence encoding the vesicular stomatitis virus envelope glycoprotein (VSV-G) linked to a (poly)peptide comprising or consisting of a cell membrane-binding domain, said nucleic acid sequence comprising in 5′ to 3′ direction (a) a first sequence segment encoding an endoplasmic reticulum (ER) signal sequence; (b) a second sequence segment encoding said (poly)peptide comprising or consisting of a cell membrane-binding domain; (c) a third sequence segment encoding a linker; and (d) a fourth sequence segment encoding said VSV-G. Further, the invention relates to a vector comprising the nucleic acid molecule of the invention, a host cell comprising said vector or nucleic acid molecule, the polypeptide encoded by said nucleic acid molecule and a method of producing the polypeptide encoded by said nucleic acid molecule. In addition, the invention relates to a pseudotyped lentiviral vector particle, a method of transducing a cell as well as a kit comprising various combinations of the nucleic acid molecule, vectors, polypeptides and host cells of the invention.
公开/授权文献
- EP3092306B1 PSEUDOTYPED LENTIVIRAL VECTORS 公开/授权日:2019-10-16
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