Embodiments of the present invention disclose a pixel structure, a display substrate and a display apparatus. The pixel structure includes a plurality of repeated units, each of the repeated units including a first portion, a second portion, a third portion, and a fourth portion which are arranged in a shape of a matrix of two rows by two columns. Each of the first through fourth portions includes four rows by four columns of sub-pixels, and includes, in each of the rows, the columns, and diagonals thereof, first through fourth sub-pixels which are different in color from one another. Two sub-pixels, which have the same color, and are in the same row in any left-right adjacent two of the first through fourth portions, are in an odd-numbered column of one of the two portions and an even-numbered column of the other of the two portions, respectively; and two sub-pixels, which have the same color, and are in the same column in any up-down adjacent two of the first through fourth portions, are in an odd-numbered row of one of the two portions and an even-numbered row of the other of the two portions, respectively.