The invention concerns (computer implemented) methods for data correction, in particular, fluorescence data, related systems, software, graphic user interfaces and the use thereof, more in particular, the invention describes a method for adjusting a part of measurements taken from a plurality of measurements, under the same time-dependent area, at different time points in which said adjustments are performed in order to obtain calculated measurements which can be compared as if they were taken at the same time point (and hence, under the same environment).