A base station and a method thereof for acquiring channel status information in a mobile communication system operating in a multiuser multiple-input multiple-output transmission mode are provided. The method includes generating and transmitting, to a terminal, interference measurement configuration information for measuring interference caused by signals transmitted from a serving base station of the terminal to at least one other terminal in the transmission mode; and receiving channel status information generated using the transmitted information from the terminal. The present disclosure relates to a communication method and system for converging a 5th-Generation communication system for supporting higher data rates beyond a 4th-Generation system with a technology for Internet of Things, and may be applied to intelligent services based on the 5G communication technology and the IoT-related technology, such as smart home, smart building, smart city, smart car, connected car, health care, digital education, smart retail, security and safety services.