Provided is a method for fastening a separation type tricycle in which a connecting pipe for connecting a front wheel part and a rear wheel part can be separated from and fastened to a head tube of the front wheel part, wherein the front wheel part and the rear wheel part are separated from each other by forming an ear-shaped groove, in which a handle bar can be put, in a right-side-open rectangular shaped plate fixed to the end of the connecting pipe so as to be used for attachment/detachment in a fastening device of the head tube, and then the end of a connecting bar for connecting the front wheel part and the rear wheel part is put on the handle bar by using the groove and another place is fixed thereto so as to set three wheels at the shortest distance therewith, thereby conveniently moving and storing the tricycle.