发明公开
EP3168300A1 YEAST EXPRESSING SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOPROCESSING USING STARCH AND CELLULOSE
审中-公开
酵母表达淀粉和纤维素用于固结生物加工的糖化酶
- 专利标题: YEAST EXPRESSING SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOPROCESSING USING STARCH AND CELLULOSE
- 专利标题(中): 酵母表达淀粉和纤维素用于固结生物加工的糖化酶
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申请号: EP16192843.7申请日: 2011-06-03
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公开(公告)号: EP3168300A1公开(公告)日: 2017-05-17
- 发明人: BREVNOVA, Elena , MCBRIDE, John, E. , WISWALL, Erin , WENGER, Kevin, S. , CAIAZZA, Nicky , HAU, Heidi, H. , ARGYROS, Aaron , AGBOGBO, Frank , RICE, Charles, F. , BARRETT, Trisha , BARDSLEY, John, S. , FOSTER, Abigail, S. , WARNER, Anne, K. , MELLON, Mark , SKINNER, Ryan , SHIKHARE, Indraneel , DEN HAAN, Riaan , GANDHI, Chhayal, V. , BELCHER, Alan , RAJGARHIA, Vineet, B. , FROEHLICH, Allan, C. , DELEAULT, Kristen, M. , STONEHOUSE, Emily , TRIPATHI, Shital, A. , GOSSELIN, Jennifer , CHIU, Yin-Ying , XU, Haowen
- 申请人: Lallemand Hungary Liquidity Management LLC , Stellenbosch University
- 申请人地址: Wesselenyi utca 16 3rd Floor Budapest 1077 HU
- 专利权人: Lallemand Hungary Liquidity Management LLC,Stellenbosch University
- 当前专利权人: Lallemand Hungary Liquidity Management LLC,Stellenbosch University
- 当前专利权人地址: Wesselenyi utca 16 3rd Floor Budapest 1077 HU
- 代理机构: Cornish, Kristina Victoria Joy
- 优先权: US351165P 20100603; US420142P 20101206
- 主分类号: C12N9/42
- IPC分类号: C12N9/42 ; C12P7/06
摘要:
The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured togetherand used to produce ethanol from biomass feedstock.
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