The invention belongs to the technical field of display, and specifically relates to a pixel structure and a display method thereof, and a display device including the pixel structure. The pixel structure comprises first pixel units and second pixel units which are sequentially arranged in staggered positions, wherein the first pixel unit comprises a first sub-pixel and a second sub-pixel arranged in an oblique line, and the second pixel unit comprises a third sub-pixel and a fourth sub-pixel arranged in an oblique line, and wherein the first sub-pixel and the third sub-pixel have different basic colors, and the second sub-pixel and the fourth sub-pixel have the same basic color. The pixel structure is capable of increasing the aperture ratios of sub-pixels while achieving high resolution, which not only simplifies the manufacture process, obtains good brightness level and extends the lifetime of product, but also prevents the error of color edge and the color nonuniformity by setting the weighted value of the missing basic color of the pixel structure in the display process, hence achieving better display effect.