Use of pycnidione, epolone A, and epolone B as anti-dandruff actives either alone or in combination in anti-dandruff compositions, particularly shampoos and conditioners. The actives are particularly effective against Malassezia yeasts and Malassezia furfur. A method of obtaining pycnidione, epolone A, and epolone B from culturing of Neosetophoma samarorum is also described.