- 专利标题: POLYMERASE COMPOSITIONS AND KITS, AND METHODS OF USING AND MAKING THE SAME
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申请号: EP16794093.1申请日: 2016-09-27
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公开(公告)号: EP3356557A2公开(公告)日: 2018-08-08
- 发明人: MAZUR, Daniel , VANDER HORN, Peter , TOZER, Eileen , CHEN, Sihong , LUO, Guobin , SHIRLEY, Joshua , HEINEMANN, Kevin
- 申请人: Life Technologies Corporation
- 申请人地址: 5823 Newton Drive Carlsbad, CA 92008 US
- 专利权人: Life Technologies Corporation
- 当前专利权人: Life Technologies Corporation
- 当前专利权人地址: 5823 Newton Drive Carlsbad, CA 92008 US
- 代理机构: Weber, Birgit
- 优先权: US201562235616P 20151001
- 国际公布: WO2017058810 20170406
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68 ; C12N9/12
摘要:
The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification. In some aspects, the disclosure relates to recombinant polymerases useful for the generation of nucleic acid libraries and/or nucleic acid templates.
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