A pixel arrangement structure, a pixel circuit, a display panel and a driving method. The pixel arrangement structure includes a first pixel unit (100) including a first sub-pixel (110), a second sub-pixel (120), a third sub-pixel (130) and a fourth sub-pixel (140); a center of the first sub-pixel (110) is coincident with a first vertex of a first virtual diamond (150); a center of the second sub-pixel (120) is coincident with a second vertex of the first virtual diamond (150); a center of the third sub-pixel (130) is coincident with a third vertex of the first virtual diamond (150); and a center of the fourth sub-pixel (140) is coincident with a fourth vertex of the first virtual diamond (150). The pixel arrangement structure, the pixel circuit, the display panel and the driving method can reduce the distance between the sub-pixels and meanwhile reduce the occupied area of the pixel circuit, and hence improve the resolution of the display panel, and can perform initializing discharge on OLEDs in the driving process, ensure the accuracy at a low gray scale and full black under full-dark-state images, and effectively improve the contrast of the entire display panel.