- 专利标题： METHOD FOR ANALYZING GLYCOSAMINOGLYCAN
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申请号： EP18757368.8申请日： 2018-02-20
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公开(公告)号： EP3588090A1公开(公告)日： 2020-01-01
- 发明人: TANAKA, Noboru , KIDA, Sachiho
- 申请人： JCR Pharmaceuticals Co., Ltd.
- 申请人地址： 3-19, Kasuga-cho Ashiya-shi Hyogo 659-0021 JP
- 专利权人： JCR Pharmaceuticals Co., Ltd.
- 当前专利权人： JCR Pharmaceuticals Co., Ltd.
- 当前专利权人地址： 3-19, Kasuga-cho Ashiya-shi Hyogo 659-0021 JP
- 代理机构： Balder IP Law, S.L.
- 优先权： JP2017030518 20170221; JP2017196506 20171010
- 国际公布： WO2018155443 20180830
- 主分类号： G01N33/66
- IPC分类号： G01N33/66 ; G01N27/62 ; G01N30/06 ; G01N30/88 ; G01N33/48
摘要：
[Problem] A method for measuring the amount of trace amo unts of heparan sulfate and/or dermatan sulfate containe d in a sample is provided.
[Solution] The present invention provides a method for decomposing heparan sulfate and dermatan sulfate contained in a sample into, respectively, a disaccharide in which uronic acid and amino sugar are α1,4-bonded and a disaccharide in which uronic acid and amino sugar are α1,3-bonded. More particularly, the present invention provides a method for decomposing heparan sulfate and dermatan sulfate by heating the heparan sulfate and the dermatan sulfate in, for example, 2,2-dimethoxypropane-containing methanol hydrochloride at 65-85°C for 80-180 minutes and 60-80°C for 20-100 minutes, respectively.
[Solution] The present invention provides a method for decomposing heparan sulfate and dermatan sulfate contained in a sample into, respectively, a disaccharide in which uronic acid and amino sugar are α1,4-bonded and a disaccharide in which uronic acid and amino sugar are α1,3-bonded. More particularly, the present invention provides a method for decomposing heparan sulfate and dermatan sulfate by heating the heparan sulfate and the dermatan sulfate in, for example, 2,2-dimethoxypropane-containing methanol hydrochloride at 65-85°C for 80-180 minutes and 60-80°C for 20-100 minutes, respectively.
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