Embodiments of the disclosure provide a gene sequencing chip, including: an upper substrate including a plurality of liquid inlets for inletting liquid drops; a lower substrate opposite to the upper substrate and spaced therefrom by a gap, the gap being provided for allowing the liquid drops to move therein, the lower substrate including a liquid drop operation region, the liquid drop operation region including a manipulation electrode array. The manipulation electrode array includes multiple first sub-arrays for preparing a gene library, multiple second sub-arrays for sequencing the prepared gene library, each first sub-array being adjacent to one of the second sub-arrays. Based on the gene sequencing chip provided in this disclosure, operations to tiny liquid drops such as movement, fusion and splitting can be accurately manipulated by using digital microfluidic techniques, and all steps of the gene sequencing from library preparation to gene sequencing can be completed in a programmatic manner on one chip. The chip has a high degree of integration, the liquid drops can be manipulated accurately, the consumption of the reagents can be reduced, and the preparation process is simple and mature.